This study aimed to investigate the properties of up-conversion luminescent nanomaterials containing Tm(III) and Yb(III) based on NaYF4 host. The materials were synthesized using the hydrothermal method. The morphology, structure and luminescent properties of the material were analyzed using field emission scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and photoluminescence spectroscopy. The results indicate that the synthesized nano-materials were in the form of particles with a diameter ranging from 100-200 nm, had a hexagonal β-NaYF4 phase structure, and emitted blue light at a wavelength of 980 nm. These luminescent properties of the material indicate their potential application in biomedical fields.