This paper is concerned with the ability of natural products such as vinegar, tea, okra, lemon juice, grapefruit peel essential oil, and baking soda to prevent mold growth on bamboo and wood materials. The results showed that grapefruit peel essential oil had the highest biological activity compared to the other products in preventing mold growth on wood materials. Additionally, a process for preventing mold growth on bamboo and wood materials was developed and the parameters were optimized using the Box-Behnken surface response correlation method. The optimal process conditions were determined by analyzing the response surface of the three-dimensional surface plot and solving the regression model equation using Design Expert software. The optimal conditions, including the ratio of product to coverage area 5/1, concentration 53%, time in 238 minutes and temperature 40^o C, have been tested and showed that the time for mold appearance was 254 days.