Experiments on the effects of melaleuca biochar on the growth of water spinach (Ipomoea aquatica Forsk) under net conditions were conducted at Can Tho University. In the light of these, melaleuca biochar has been adsorbed with nutrients from biogas and utilized in various dosages as a chemical fertilizer replacement for water spinach. Water spinach plants were fertilized with a combination of 25% chemical fertilizers and melaleuca biochar, which absorbed nutrients from biogas. As a result, when compared to chemical fertilizer treatment, they achieved the highest value in terms of plant height (38.6 cm), an average number of leaves (10 leaves), fresh biomass (16.23 g pot-1), and dry biomass (1.21 g pot-1). Furthermore, the nitrate concentration of the vegetable in (NT5) met international quality and safety standards (FAO/WHO, 2002). Henceforth, using melaleuca biochar with chemical fertilizers was able to boost crop productivity is a realistic choice.